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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in lots of tumors, which results in chromosome instability, and therefore may possibly add to tumorigenesis. This protein was discovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important part in marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are described. Two supplemental splice variants had been documented but comprehensive nucleotide sequence info just isn't however offered.
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Blocking peptides are peptides that bind especially towards the goal antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged from the antibody.
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This guideline outlines further possible optimization of cellular and immunoassay parameters to guarantee the very best outcomes are...
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This manual outlines more attainable optimization of cellular and immunoassay parameters to be certain the absolute best outcomes are...
Antibodies bound to the blocking peptide no longer bind into the epitope to the goal protein. By evaluating the staining from the blocked antibody versus the antibody on your own, you can see which staining is unique.
Focus on Info Cyclin E belongs on the very conserved cyclin relatives, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation designs which lead into the temporal coordination of each and every mitotic celebration. Cyclin E types a posh with and capabilities being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates at the G1-S phase boundary which is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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